Apsilon™ Nasal Swab DNA/RNA Extraction Kit

Apsilon™ Nasal Swab DNA/RNA Extraction Kit provides a unique and rapid method to extract and purify both DNA and RNA in 10 mins from nasal swab samples. It extracts nucleic acid effectively from a variety of microorganisms, including viruses and bacteria (gram-positive and gram-negative). This kit, which utilizes magnetic beads-based technology, is compatible with automated platforms to enable high throughput extraction in extremely short processing time. Reagents are specially formulated to be enzyme-free, alcohol-free, and stable when stored at room temperature, thus eliminating any special storage and handling requirements.

Comparison with Leading Commercial Kits:

Better or comparable performance compared with other commercial kits.

Nucleic acid extractions from S. aureus (gram-positive bacteria) and influenza A (RNA virus) at indicated concentrations with Apsilon™ kit and leading commercial kits. Protocols were followed according to manufacturers' instructions. Both LAMP and qPCR performances were evaluated, and Apsilon™ kit is better or comparable to other commercial kits. (For LAMP/RT-LAMP, Cq refers to threshold time and each cycle is 30 sec.)

High quality nucleic acid obtained from our kit contain no PCR inhibitors.

Nucleic acid extractions from 300 μL pathogen-spiked swab matrixes were performed with Apsilon™ Nasal Swab DNA/RNA Extraction Kit. qPCR/RT-qPCR performances of undiluted, 10x-diluted and 100x-diluted eluates indicate there is little or no PCR inhibition.

Different nucleic acids are extracted simultaneously from our kit.

Nucleic acid extraction from 300 μL swab matrix spiked with 1000x LoD each of S. aureus (gram-positive bacteria) and HCoV-OC43 (RNA virus) was performed with Apsilon™ Nasal Swab DNA/RNA Extraction Kit. A total of 3 replicates were done, and similar results were obtained amongst the replicates. Both targets showed good qPCR/RT-qPCR amplification.

Our kit is automation-compatible with better or comparable recovery rates.

Nucleic acid extraction of varying amounts of SARS-CoV-2 (RNA virus) was done with both Apsilon™ and leading commercial kit. Apsilon™ kit extraction was performed with Katsura M32 Nucleic Acid Extractor, while manual extraction was performed for Q kit. Based on standard curve generated from SARS-CoV-2 RNA spiked directly into RT-qPCR, the RNA yield from each kit was quantitated, and recovery rates were tabulated. Apsilon™ kit has better or comparable recovery rates to that of Q kit.

Email us at SGDRC.APSILON@deltaww.com

Empower your lab with the Apsilon™ Extraction Kit – a fast, ready-to-use solution that streamlines your workflow for greater efficiency, accuracy, and confidence. Get in touch today to see how we can help transform your lab operations.