Apsilon™ Whole Blood DNA/RNA Extraction Kit (Host & Pathogen)

Apsilon™ Whole Blood DNA/RNA Extraction Kit (Host & Pathogen) provides a unique and rapid method to extract and purify both DNA and RNA in around 13 mins, from hosts as well as a variety of microorganisms, including viruses, bacteria (gram-negative and gram-positive) and fungi, from whole blood samples. This kit, which utilizes magnetic beads-based technology, is compatible with automated platforms to enable high throughput extraction in extremely short processing time. Reagents are specially formulated to be enzyme-free, alcohol-free and stable when stored at room temperature, thus eliminating any special storage and handling requirements.

Comparison with Leading Commercial Kits:

Better or comparable performance compared with other commercial kits.

Nucleic acid extractions from blood spiked with either S. aureus (gram-positive bacteria), dengue virus Type 2 (dengue 2; RNA virus), or Candida albicans (C. albicans; fungus) at 1.5x LoD with Apsilon™ kit and leading commercial kits. Protocols were followed according to manufacturers' instructions. Both LAMP and qPCR performances were evaluated, and Apsilon™ kit is better or comparable to other commercial kits. (*No amplification observed. For LAMP/RT-LAMP, Cq refers to threshold time and each cycle is 30 sec.)

High quality nucleic acid obtained from our kit contain no PCR inhibitors.

Nucleic acid extraction from 300 μL blood spiked with 100 TCID50 dengue virus Type 2 (dengue 2; RNA virus) was performed with Apsilon™ Whole Blood DNA/RNA Extraction Kit (Host & Pathogen). RT-qPCR performances of undiluted, 10x-diluted and 100x-diluted eluates indicate there is little or no PCR inhibition.

Different nucleic acids are extracted simultaneously with our kit (Host & Pathogen).

Nucleic acid extractions from 300 μL blood spiked with either 15 CFU of S. aureus (gram-positive bacteria), 1000 TCID50 dengue virus Type 2 (dengue 2; RNA virus), or both pathogens were performed with Apsilon™ Whole Blood DNA/RNA Extraction Kit (Host & Pathogen). Similar qPCR/RT-qPCR amplifications were observed between samples spiked with only one or both pathogens, indicating that the kit allows simultaneous extraction of both RNA and DNA from the same sample.

Apsilon™ kit has comparable performance to other leading commercial kits.

Nucleic acid extraction of whole blood was done in duplicates with Apsilon™ and leading commercial kits. GAPDH qPCR was then performed on the eluates as an approximation of extracted blood gDNA amount, and the results indicate that Apsilon™ kit has similar performance as the other kits tested.

Email us at SGDRC.APSILON@deltaww.com

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